21Shares Polkadot ETF S-1/A

AMENDMENT NO. 6 TO FORM S-1

TRUST AGREEMENT

SECOND AMENDED AND RESTATED TRUST AGREEMENT

FORM OF THIRD AMENDED AND RESTATED TRUST AGREEMENT

CERTIFICATE OF TRUST

CERTIFICATE OF AMENDMENT TO CERTIFICATE OF TRUST

CERTIFICATE OF AMENDMENT TO CERTIFICATE OF TRUST

OPINION OF DECHERT LLP AS TO LEGALITY

TAX OPINION OF DECHERT LLP

FORM OF SPONSOR AGREEMENT

FORM OF AUTHORIZED PARTICIPANT AGREEMENT (TYPE A)

FORM OF AUTHORIZED PARTICIPANT AGREEMENT (TYPE B)

FORM OF PRIME BROKER AGREEMENT

FORM OF BITGO CUSTODIAL SERVICES AGREEMENT

FORM OF FUND ADMINISTRATION AND ACCOUNTING AGREEMENT

FORM OF TRANSFER AGENCY AND SERVICES AGREEMENT

FORM OF PRICING BENCHMARK LICENSING AGREEMENT

FORM OF CASH CUSTODY AGREEMENT

FORM OF MARKETING AGENT AGREEMENT

WEB 3.0 TECHNOLOGIES FOUNDATION SUBSCRIPTION AGREEMENT

FORM OF INITIAL SEED CREATION SUBSCRIPTION AGREEMENT

FORM OF MASTER INFRASTRUCTURE AS A SERVICE AGREEMENT

CONSENT OF INDEPENDENT REGISTERED PUBLIC ACCOUNTING FIRM

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